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Intron-mediated enhancement
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Intron-mediated enhancement : ウィキペディア英語版
Intron-mediated enhancement
Intron-mediated enhancement (IME) is the ability of an intron sequence to enhance the expression of a gene containing that intron. The term was first defined in a 1990 publication that investigated the enhancing effects of two specific maize introns.
Since then there have been a number of publications that have investigated this phenomenon, though the mechanism(s) by which IME works are still not completely understood. When testing to see whether any given intron produces an IME effect, it is typical to compare the expression of two constructs, one containing the intron and one without it, and to express the difference between the two results as a "fold increase" in enhancement.
Not all introns enhance gene expression, but those that do typically enhance expression between 2– and 10–fold relative to an intronless control. Introns that enhance expression have been detected in humans, mice, ''Arabidopsis'', rice, and ''C. elegans''.
==References==


抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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